Fine mapping and candidate gene identification of a recessive genic male sterility gene (Bnms3) in rapeseed
نویسندگان
چکیده
On the basis of the previous mapping study for male sterility gene Bnms3, we further narrowed it from a genomic region of 470kb to 93.7kb, using a large population consisting of 1339 fertile and 1151 sterile plants, and locus specific markers derived from Brassica rapa of A10. Seventeen open reading frames (ORFs) were predicated in the target region. Of them, two most likely candidate genes were proposed. Five co-dominant markers flanking Bnms3 with 0.00cM to 0.15cM in distance were developed, which are available to distinguish three genotypes of Ms3Ms3, Ms3ms3 and ms3ms3. Allelic distribution of Bnms3 locus among 96 genetically diverse cultivars/lines showed all the materials carrying homozygous Ms3 allele (Ms3Ms3) in Bnms3 locus, which might explain the phenomenon for why REGMS could be restored by almost all the rapeseed cultivars/lines.
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